Group 7 project

Cell-type and brain region-resolved mouse proteome - Sharma et al. 2015

Introduction

• Goal

  • Can we reliably predict protein abundance from transcript expression levels, or vice versa?

• Data

  • Cultured cell types from mice brain

  • Proteomics and transcriptomics data

Results

Transcriptome density analysis

Data validation: replicates correlation

Correlation Heatmap

LogFold change across Cell types

Rank-Rank analysis across data types

Principal Component Analysis

Conclusion

• Transcriptomics ≠ Proteomics

  • Most abundant transcripts and the most abundant proteins often do not correlate

• Proteomics provides valuable insights

  • Different proteomic profiles depending on cell type / brain region

• Why is this important?

  • mRNA results don’t translate into real world proteomic profiles