Setup knitr and load utility functions

knitr::opts_chunk$set(echo = TRUE)
knitr::opts_knit$set(root.dir="E:/DISC/reproducibility")
utilities_path = "./source/utilities.r"
source(utilities_path)
Registered S3 method overwritten by 'dplyr':
  method           from
  print.rowwise_df     
Registered S3 method overwritten by 'htmlwidgets':
  method           from         
  print.htmlwidget tools:rstudio
Registered S3 method overwritten by 'data.table':
  method           from
  print.data.table     
reldist: Relative Distribution Methods
Version 1.6-6 created on 2016-10-07.
copyright (c) 2003, Mark S. Handcock, University of California-Los Angeles
 For citation information, type citation("reldist").
 Type help(package="reldist") to get started.
library(limma)

Settings

method_name = c("Raw", "DISC", "scVI", "MAGIC", "DCA", "scScope", "DeepImpute", "VIPER", "scImpute")
method_color = c("#A5A5A5", "#E83828", "#278BC4", "#EADE36", "#198B41", "#920783", "#F8B62D", "#8E5E32", "#1E2B68")
text_color = rep("black", length(method_name))
names(text_color) = method_name
text_color["DISC"] = "red"
options(future.globals.maxSize= 4.5e9)

Identify reference DEGs from bulk data.

gene_bulk_mat = readh5_loom("./data/JURKAT_293T/bulk.loom")
[1] "./data/JURKAT_293T/bulk.loom"
[1] 58011     4
gene_bulk_filt0 = round(gene_bulk_mat[!grepl('^MT-', rownames(gene_bulk_mat)), ])
gene_bulk_filt1 = gene_bulk_filt0[rowSums(gene_bulk_filt0 > 0) > 0, ]
bulk_sample_cell_type = sapply(colnames(gene_bulk_filt1), function(x){
  return(strsplit(x, "_", fixed = T)[[1]][[2]])
})
design_mat = cbind(1, ifelse(bulk_sample_cell_type == bulk_sample_cell_type[1], 1, 0))
fit <- eBayes(lmFit(voom(gene_bulk_filt1, design = design_mat), design = design_mat))
bulk_DE_matrix = topTable(fit, coef = 2, number = nrow(gene_bulk_filt1))
bulk_DEG = rownames(bulk_DE_matrix)[abs(bulk_DE_matrix[, "logFC"]) >= 1.5 & bulk_DE_matrix[, "adj.P.Val"] <= 0.05]
print(head(bulk_DE_matrix))
print(head(bulk_DEG))
[1] "XIST"   "TMSB4X" "BCL11B" "TCF7"   "NEFM"   "PRKCB" 

Identify DEGs using imputed results.

if(!file.exists("./results/JURKAT_293T/sc_DEG_list.rds")){
  dir.create("./results/JURKAT_293T", showWarnings = F, recursive = T)
  gene_bc_mat = readh5_loom("./data/JURKAT_293T/raw.loom")
  cell_293T = colnames(gene_bc_mat)[grep("293T", colnames(gene_bc_mat))]
  cell_Jurkat = colnames(gene_bc_mat)[grep("JURKAT", colnames(gene_bc_mat))]
  cell_type = c(rep("293T", length(cell_293T)), rep("Jurkat", length(cell_Jurkat)))
  names(cell_type) = c(cell_293T, cell_Jurkat)
  cell_type = cell_type[colnames(gene_bc_mat)]
  cell_type = factor(cell_type, levels = sort(unique(cell_type)))
  gene_filter = gene_selection(gene_bc_mat, 10)
  sc_gene = rownames(gene_bc_mat)[gene_filter]
  sc_DEG_list = list(wilcox = list(), MAST = list())
  for(ii in method_name){
    if(ii == "Raw"){
      gene_bc_filt = gene_bc_mat[sc_gene, ]
    }else{
      gene_bc_mat = readh5_loom(paste0("./data/JURKAT_293T/", ii, "_JURKAT_293T.loom"))
      gene_bc_filt = gene_bc_mat[sc_gene, ]
    }
    seurat_obj = CreateSeuratObject(counts = as.data.frame(gene_bc_filt), min.cells = 0, min.features = 0)
    seurat_obj = NormalizeData(seurat_obj, normalization.method = "LogNormalize", scale.factor = 10000)
    seurat_obj@active.ident = cell_type
    suppressWarnings({sc_DEG_list[["wilcox"]][[ii]] = FindMarkers(seurat_obj, ident.1 = "293T", ident.2 = "Jurkat", min.pct = 0.1, logfc.threshold = 0, test.use = "wilcox", verbose = F)})
    suppressWarnings({sc_DEG_list[["MAST"]][[ii]] = FindMarkers(seurat_obj, ident.1 = "293T", ident.2 = "Jurkat", min.pct = 0.1, logfc.threshold = 0, test.use = "MAST", verbose = F)})
    print(ii)
  }
  saveRDS(sc_DEG_list, paste0("./results/JURKAT_293T/sc_DEG_list.rds"))
}else{
  sc_DEG_list = readRDS(paste0("./results/JURKAT_293T/sc_DEG_list.rds"))
}
[1] "./data/JURKAT_293T/raw.loom"
[1] 32738  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "Raw"
[1] "./data/JURKAT_293T/DISC_JURKAT_293T.loom"
[1] 32738  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "DISC"
[1] "./data/JURKAT_293T/scVI_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "scVI"
[1] "./data/JURKAT_293T/MAGIC_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "MAGIC"
[1] "./data/JURKAT_293T/DCA_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "DCA"
[1] "./data/JURKAT_293T/scScope_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "scScope"
[1] "./data/JURKAT_293T/DeepImpute_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "DeepImpute"
[1] "./data/JURKAT_293T/VIPER_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "VIPER"
[1] "./data/JURKAT_293T/scImpute_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "scImpute"

False detected DEG test.

if(!file.exists("./results/JURKAT_293T/sc_false_DEG_list.rds")){
  dir.create("./results/JURKAT_293T", showWarnings = F, recursive = T)
  gene_bc_mat = readh5_loom("./data/JURKAT_293T/raw.loom")
  gene_filter = gene_selection(gene_bc_mat, 10)
  sc_gene = rownames(gene_bc_mat)[gene_filter]
  cell_293T = colnames(gene_bc_mat)[grep("293T", colnames(gene_bc_mat))]
  df = expand.grid(c(10, 50, 100, 500), c(10, 50, 100, 500))
  colnames(df) = c("n1", "n2")
  df = df[df[, "n1"] <= df[, "n2"], ]
  sc_false_DEG_list = list(wilcox = list(), MAST = list())
  for(ii in seq(nrow(df))){
    cn1 = df[ii, "n1"]
    cn2 = df[ii, "n2"]
    set.seed(12345)
    id = sample(seq(length(cell_293T)), cn1 + cn2)
    this_name = paste0(cn1, '_', cn2)
    for(jj in method_name){
      if(jj == "Raw"){
        expr = gene_bc_mat[sc_gene, cell_293T][, id]
      }else{
        gene_bc_mat = readh5_loom(paste0("./data/JURKAT_293T/", jj, "_JURKAT_293T.loom"))
        expr = gene_bc_mat[sc_gene, cell_293T][, id]
      }
      if(jj == method_name[1]){
        sc_false_DEG_list[["wilcox"]][[jj]] = list()
        sc_false_DEG_list[["MAST"]][[jj]] = list()
      }
      seurat_obj = CreateSeuratObject(counts = as.data.frame(expr), min.cells = 0, min.features = 0)
      seurat_obj = NormalizeData(seurat_obj, normalization.method = "LogNormalize", scale.factor = 10000)
      cell_type = c(rep("cn1", cn1),rep("cn2", cn2))
      names(cell_type) = colnames(expr)
      seurat_obj@active.ident = factor(cell_type, levels = sort(unique(cell_type)))
      suppressWarnings({sc_false_DEG_list[["wilcox"]][[jj]][[this_name]] = FindMarkers(seurat_obj, ident.1 = "cn1", ident.2 = "cn2", min.pct = 0.1, logfc.threshold = 0, test.use = "wilcox", verbose = F)})
      suppressWarnings({sc_false_DEG_list[["MAST"]][[jj]][[this_name]] = FindMarkers(seurat_obj, ident.1 = "cn1", ident.2 = "cn2", min.pct = 0.1, logfc.threshold = 0, test.use = "MAST", verbose = F)})
    }
    print(this_name)
  }
  saveRDS(sc_false_DEG_list, paste0("./results/JURKAT_293T/sc_false_DEG_list.rds"))
}else{
  sc_false_DEG_list = readRDS(paste0("./results/JURKAT_293T/sc_false_DEG_list.rds"))
}
[1] "./data/JURKAT_293T/raw.loom"
[1] 32738  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/DISC_JURKAT_293T.loom"
[1] 32738  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/scVI_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/MAGIC_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/DCA_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/scScope_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/DeepImpute_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/VIPER_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/scImpute_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "10_10"
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/DISC_JURKAT_293T.loom"
[1] 32738  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/scVI_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/MAGIC_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/DCA_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/scScope_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/DeepImpute_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/VIPER_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/scImpute_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "10_50"
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/DISC_JURKAT_293T.loom"
[1] 32738  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/scVI_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/MAGIC_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/DCA_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/scScope_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/DeepImpute_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/VIPER_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/scImpute_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "50_50"
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/DISC_JURKAT_293T.loom"
[1] 32738  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/scVI_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/MAGIC_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/DCA_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/scScope_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/DeepImpute_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/VIPER_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/scImpute_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "10_100"
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/DISC_JURKAT_293T.loom"
[1] 32738  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/scVI_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/MAGIC_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/DCA_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/scScope_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/DeepImpute_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/VIPER_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/scImpute_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "50_100"
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/DISC_JURKAT_293T.loom"
[1] 32738  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/scVI_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/MAGIC_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/DCA_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/scScope_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/DeepImpute_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/VIPER_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/scImpute_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "100_100"
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/DISC_JURKAT_293T.loom"
[1] 32738  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/scVI_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/MAGIC_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/DCA_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/scScope_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/DeepImpute_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/VIPER_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/scImpute_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "10_500"
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/DISC_JURKAT_293T.loom"
[1] 32738  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/scVI_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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 Completed [===================================================================================================] 100% with 0 failures
                                                                                                                                     

Done!
[1] "./data/JURKAT_293T/MAGIC_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

 Completed [---------------------------------------------------------------------------------------------------]   0% with 0 failures
 Completed [---------------------------------------------------------------------------------------------------]   1% with 0 failures
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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/DCA_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/scScope_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/DeepImpute_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/VIPER_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/scImpute_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "50_500"
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/DISC_JURKAT_293T.loom"
[1] 32738  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/scVI_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/MAGIC_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/DCA_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/scScope_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/DeepImpute_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/VIPER_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/scImpute_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "100_500"
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/DISC_JURKAT_293T.loom"
[1] 32738  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/scVI_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/MAGIC_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/DCA_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/scScope_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/DeepImpute_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

 Completed [---------------------------------------------------------------------------------------------------]   0% with 0 failures
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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/VIPER_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "./data/JURKAT_293T/scImpute_JURKAT_293T.loom"
[1] 13328  6143
Feature names cannot have underscores ('_'), replacing with dashes ('-')Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Assuming data assay in position 1, with name et is log-transformed.

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Done!
Combining coefficients and standard errors
Calculating log-fold changes
Calculating likelihood ratio tests
Refitting on reduced model...

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Done!
[1] "500_500"
overlap_wilcox = sapply(sc_DEG_list[["wilcox"]], function(x){
  return(mean(sapply(seq(100) * 10, function(y){
    return(mean(rownames(x[order(x[, "p_val_adj"]), ])[seq(y)] %in% bulk_DEG))
  })))
})

overlap_MAST = sapply(sc_DEG_list[["MAST"]], function(x){
  return(mean(sapply(seq(100) * 10, function(y){
    return(mean(rownames(x)[seq(y)] %in% bulk_DEG))
  })))
})
print(overlap_MAST)
       Raw       DISC       scVI      MAGIC        DCA    scScope DeepImpute      VIPER   scImpute 
 0.6084401  0.6891465  0.5364339  0.4615233  0.5467916  0.6190999  0.5231432  0.5919032  0.6029579 
print(overlap_wilcox)
       Raw       DISC       scVI      MAGIC        DCA    scScope DeepImpute      VIPER   scImpute 
 0.5883429  0.6877088  0.5364339  0.4615233  0.5467488  0.5944116  0.4874671  0.5759028  0.5910734 

Note: This result has tiny difference from our paper as the sample cell is different.

DEG_wilcox_false = sapply(sc_false_DEG_list[["wilcox"]], function(x){
  return(sapply(x, function(y){
    return(sum(y[, "p_val"] <= 0.01 & abs(y[, "avg_logFC"]) >= 0.25))
  }))
})
wilcox_false_mean = colMeans(DEG_wilcox_false)
print(wilcox_false_mean)
       Raw       DISC       scVI      MAGIC        DCA    scScope DeepImpute      VIPER   scImpute 
      21.7        4.8        0.0        0.0        0.0       18.7       10.4       12.6       11.3 
DEG_MAST_false = sapply(sc_false_DEG_list[["MAST"]], function(x){
  return(sapply(x, function(y){
    return(sum(y[, "p_val"] <= 0.01 & abs(y[, "avg_logFC"]) >= 0.25))
  }))
})
MAST_false_mean = colMeans(DEG_MAST_false)
print(MAST_false_mean)
       Raw       DISC       scVI      MAGIC        DCA    scScope DeepImpute      VIPER   scImpute 
      18.7        2.4        0.0        0.0        0.0       20.8        9.9       12.1        9.9 
plot_df = data.frame(omo = (1 - overlap_wilcox[method_name]), fm = wilcox_false_mean[method_name], method = method_name)
ggplot(plot_df, aes(x = fm, y = omo, label = method, color = method)) + geom_point() + geom_text_repel() + theme_bw() + theme(legend.position = "none") + xlab("False DEG (293T)") + ylab("1 - Overlap") + ggtitle("wilcox") + scale_colour_manual(values = method_color)

plot_df = data.frame(omo = (1 - overlap_MAST[method_name]), fm = MAST_false_mean[method_name], method = method_name)
ggplot(plot_df, aes(x = fm, y = omo, label = method, color = method)) + geom_point() + geom_text_repel() + theme_bw() + theme(legend.position = "none") + xlab("False DEG (293T)") + ylab("1 - Overlap") + ggtitle("MAST") + scale_colour_manual(values = method_color)

---
title: "DEG Identification"
output: html_notebook
---
### Setup knitr and load utility functions
```{r setup}
knitr::opts_chunk$set(echo = TRUE)
knitr::opts_knit$set(root.dir="E:/DISC/reproducibility")
```
```{r}
utilities_path = "./source/utilities.r"
source(utilities_path)
library(limma)
```
### Settings
```{r}
method_name = c("Raw", "DISC", "scVI", "MAGIC", "DCA", "scScope", "DeepImpute", "VIPER", "scImpute")
method_color = c("#A5A5A5", "#E83828", "#278BC4", "#EADE36", "#198B41", "#920783", "#F8B62D", "#8E5E32", "#1E2B68")
names(method_color) = method_name
text_color = rep("black", length(method_name))
names(text_color) = method_name
text_color["DISC"] = "red"
options(future.globals.maxSize= 4.5e9)
```
### Identify reference DEGs from bulk data.
```{r}
gene_bulk_mat = readh5_loom("./data/JURKAT_293T/bulk.loom")
gene_bulk_filt0 = round(gene_bulk_mat[!grepl('^MT-', rownames(gene_bulk_mat)), ])
gene_bulk_filt1 = gene_bulk_filt0[rowSums(gene_bulk_filt0 > 0) > 0, ]
bulk_sample_cell_type = sapply(colnames(gene_bulk_filt1), function(x){
  return(strsplit(x, "_", fixed = T)[[1]][[2]])
})
design_mat = cbind(1, ifelse(bulk_sample_cell_type == bulk_sample_cell_type[1], 1, 0))
fit <- eBayes(lmFit(voom(gene_bulk_filt1, design = design_mat), design = design_mat))
bulk_DE_matrix = topTable(fit, coef = 2, number = nrow(gene_bulk_filt1))
bulk_DEG = rownames(bulk_DE_matrix)[abs(bulk_DE_matrix[, "logFC"]) >= 1.5 & bulk_DE_matrix[, "adj.P.Val"] < 0.05]
print(head(bulk_DE_matrix))
print(head(bulk_DEG))
```
### Identify DEGs using imputed results.
```{r}
if(!file.exists("./results/JURKAT_293T/sc_DEG_list.rds")){
  dir.create("./results/JURKAT_293T", showWarnings = F, recursive = T)
  gene_bc_mat = readh5_loom("./data/JURKAT_293T/raw.loom")
  cell_293T = colnames(gene_bc_mat)[grep("293T", colnames(gene_bc_mat))]
  cell_Jurkat = colnames(gene_bc_mat)[grep("JURKAT", colnames(gene_bc_mat))]
  cell_type = c(rep("293T", length(cell_293T)), rep("Jurkat", length(cell_Jurkat)))
  names(cell_type) = c(cell_293T, cell_Jurkat)
  cell_type = cell_type[colnames(gene_bc_mat)]
  cell_type = factor(cell_type, levels = sort(unique(cell_type)))
  gene_filter = gene_selection(gene_bc_mat, 10)
  sc_gene = rownames(gene_bc_mat)[gene_filter]
  sc_DEG_list = list(wilcox = list(), MAST = list())
  for(ii in method_name){
    if(ii == "Raw"){
      gene_bc_filt = gene_bc_mat[sc_gene, ]
    }else{
      gene_bc_mat = readh5_loom(paste0("./data/JURKAT_293T/", ii, "_JURKAT_293T.loom"))
      gene_bc_filt = gene_bc_mat[sc_gene, ]
    }
    seurat_obj = CreateSeuratObject(counts = as.data.frame(gene_bc_filt), min.cells = 0, min.features = 0)
    seurat_obj = NormalizeData(seurat_obj, normalization.method = "LogNormalize", scale.factor = 10000)
    seurat_obj@active.ident = cell_type
    suppressWarnings({sc_DEG_list[["wilcox"]][[ii]] = FindMarkers(seurat_obj, ident.1 = "293T", ident.2 = "Jurkat", min.pct = 0.1, logfc.threshold = 0, test.use = "wilcox", verbose = F)})
    suppressWarnings({sc_DEG_list[["MAST"]][[ii]] = FindMarkers(seurat_obj, ident.1 = "293T", ident.2 = "Jurkat", min.pct = 0.1, logfc.threshold = 0, test.use = "MAST", verbose = F)})
    print(ii)
  }
  saveRDS(sc_DEG_list, paste0("./results/JURKAT_293T/sc_DEG_list.rds"))
}else{
  sc_DEG_list = readRDS(paste0("./results/JURKAT_293T/sc_DEG_list.rds"))
}
```
### False detected DEG test.
```{r}
if(!file.exists("./results/JURKAT_293T/sc_false_DEG_list.rds")){
  dir.create("./results/JURKAT_293T", showWarnings = F, recursive = T)
  gene_bc_mat = readh5_loom("./data/JURKAT_293T/raw.loom")
  gene_filter = gene_selection(gene_bc_mat, 10)
  sc_gene = rownames(gene_bc_mat)[gene_filter]
  cell_293T = colnames(gene_bc_mat)[grep("293T", colnames(gene_bc_mat))]
  df = expand.grid(c(10, 50, 100, 500), c(10, 50, 100, 500))
  colnames(df) = c("n1", "n2")
  df = df[df[, "n1"] <= df[, "n2"], ]
  sc_false_DEG_list = list(wilcox = list(), MAST = list())
  for(ii in seq(nrow(df))){
    cn1 = df[ii, "n1"]
    cn2 = df[ii, "n2"]
    set.seed(12345)
    id = sample(seq(length(cell_293T)), cn1 + cn2)
    this_name = paste0(cn1, '_', cn2)
    for(jj in method_name){
      if(jj == "Raw"){
        expr = gene_bc_mat[sc_gene, cell_293T][, id]
      }else{
        gene_bc_mat = readh5_loom(paste0("./data/JURKAT_293T/", jj, "_JURKAT_293T.loom"))
        expr = gene_bc_mat[sc_gene, cell_293T][, id]
      }
      if(ii == 1 & jj == method_name[1]){
        sc_false_DEG_list[["wilcox"]][[jj]] = list()
        sc_false_DEG_list[["MAST"]][[jj]] = list()
      }
      seurat_obj = CreateSeuratObject(counts = as.data.frame(expr), min.cells = 0, min.features = 0)
      seurat_obj = NormalizeData(seurat_obj, normalization.method = "LogNormalize", scale.factor = 10000)
      cell_type = c(rep("cn1", cn1),rep("cn2", cn2))
      names(cell_type) = colnames(expr)
      seurat_obj@active.ident = factor(cell_type, levels = sort(unique(cell_type)))
      suppressWarnings({sc_false_DEG_list[["wilcox"]][[jj]][[this_name]] = FindMarkers(seurat_obj, ident.1 = "cn1", ident.2 = "cn2", min.pct = 0.1, logfc.threshold = 0, test.use = "wilcox", verbose = F)})
      suppressWarnings({sc_false_DEG_list[["MAST"]][[jj]][[this_name]] = FindMarkers(seurat_obj, ident.1 = "cn1", ident.2 = "cn2", min.pct = 0.1, logfc.threshold = 0, test.use = "MAST", verbose = F)})
    }
    print(this_name)
  }
  saveRDS(sc_false_DEG_list, paste0("./results/JURKAT_293T/sc_false_DEG_list.rds"))
}else{
  sc_false_DEG_list = readRDS(paste0("./results/JURKAT_293T/sc_false_DEG_list.rds"))
}
```
```{r}
#  Note: This result has tiny difference from our paper as the sample cell is different.
overlap_wilcox = sapply(sc_DEG_list[["wilcox"]], function(x){
  return(mean(sapply(seq(100) * 10, function(y){
    return(mean(rownames(x[order(x[, "p_val_adj"]), ])[seq(y)] %in% bulk_DEG))
  })))
})

overlap_MAST = sapply(sc_DEG_list[["MAST"]], function(x){
  return(mean(sapply(seq(100) * 10, function(y){
    return(mean(rownames(x)[seq(y)] %in% bulk_DEG))
  })))
})
print(overlap_MAST)
print(overlap_wilcox)
```
Note: This result has tiny difference from our paper as the sample cell is different. 
```{r}
DEG_wilcox_false = sapply(sc_false_DEG_list[["wilcox"]], function(x){
  return(sapply(x, function(y){
    return(sum(y[, "p_val"] <= 0.01 & abs(y[, "avg_logFC"]) >= 0.25))
  }))
})
wilcox_false_mean = colMeans(DEG_wilcox_false)
print(wilcox_false_mean)
DEG_MAST_false = sapply(sc_false_DEG_list[["MAST"]], function(x){
  return(sapply(x, function(y){
    return(sum(y[, "p_val"] <= 0.01 & abs(y[, "avg_logFC"]) >= 0.25))
  }))
})
MAST_false_mean = colMeans(DEG_MAST_false)
print(MAST_false_mean)
```

```{r fig.height=3, fig.width=3}
library(ggrepel)
plot_df = data.frame(omo = (1 - overlap_wilcox[method_name]), fm = wilcox_false_mean[method_name], method = method_name)
ggplot(plot_df, aes(x = fm, y = omo, label = method, color = method)) + geom_point() + geom_text_repel() + theme_bw() + theme(legend.position = "none") + xlab("False DEG (293T)") + ylab("1 - Overlap") + ggtitle("wilcox") + scale_colour_manual(values = method_color)
plot_df = data.frame(omo = (1 - overlap_MAST[method_name]), fm = MAST_false_mean[method_name], method = method_name)
ggplot(plot_df, aes(x = fm, y = omo, label = method, color = method)) + geom_point() + geom_text_repel() + theme_bw() + theme(legend.position = "none") + xlab("False DEG (293T)") + ylab("1 - Overlap") + ggtitle("MAST") + scale_colour_manual(values = method_color)
```








