This plot uses a beta-binomial distribution to plot the probability of sequencing an allele given its variant allele frequency (VAF) and the depth of coverage
After setting your target VAF and coverage, mouse-over the plot and the y-axis number holds the probability
any given allele will have at least that much x-axis coverage.
For example, at VAF=0.1 and Coverage=100, there is a 76.3% chance an allele will be supported by ≥7 reads.
Alternatively, the probability reflects the percent of alleles one can expect to be supported with ≥Min Reads.
| Label | VAF Min | VAF Max | Min Reads | Coverage | Remove |
|---|---|---|---|---|---|
| X |
+ Add Row
For a range of VAFs, we can average the probabilities to estimate the percent of alleles which should be
found given a coverage and minimum number of reads. Bins are [Min, Max). These probabilities are by default a
weighted averaged based on the SMaHT MIMS benchmark SVs, which has e.g. more alleles with VAF ≤0.02 than between
0.02 and 0.1. Turn off the checkbox to assume a uniform distribution of VAFs.